4NLG
Y-family DNA polymerase chimera Dbh-Dpo4(243-245)-Dbh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.193, 103.067, 53.049 |
| Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
| Resolution | 28.696 - 2.400 |
| R-factor | 0.1769 |
| Rwork | 0.174 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f4y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.180 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.044 | 0.191 |
| Number of reflections | 23003 | |
| <I/σ(I)> | 23.2 | 2.9 |
| Completeness [%] | 91.7 | 80.7 |
| Redundancy | 2.7 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 14% PEG3350, 0.1 M calcium acetate, 5% glycerol, 0.25 M sucrose, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
