4NL3
Crystal Structure of Listeria monocytogenes Hfq in complex with U6 RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-29 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 124.028, 123.934, 67.595 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 45.675 - 3.100 |
| R-factor | 0.2242 |
| Rwork | 0.221 |
| R-free | 0.28270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nl2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.182 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
| High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
| Rmerge | 0.114 | 0.041 | 0.303 |
| Number of reflections | 17828 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 95.9 | 98.1 | 70.5 |
| Redundancy | 3.3 | 3.3 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 40% 1,2-propanediol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
