4NL2

Crystal Structure of Listeria monocytogenes Hfq

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-05-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 2 21
Unit cell lengths	63.600, 66.850, 106.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.078 - 2.600
R-factor	0.2159
Rwork	0.213
R-free	0.27180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.481
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.15)
Phasing software	PHASER (2.5.1)
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	106.510	46.078	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.067	0.470
Total number of observations		2690	12056
Number of reflections	14557
<I/σ(I)>	10.4	6.5	1.6
Completeness [%]	100.0	99.5	100
Redundancy	5.8	5.1	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	40% 1,2-propanediol, 100 mM HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K