4NKM
Crystal structure of engineered anti-EE scFv antibody fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.265, 92.257, 142.708 |
| Unit cell angles | 90.00, 110.86, 90.00 |
Refinement procedure
| Resolution | 46.100 - 3.710 |
| R-factor | 0.2645 |
| Rwork | 0.264 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nn8 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.867 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 3.840 |
| High resolution limit [Å] | 3.710 | 3.710 |
| Rmerge | 0.221 | |
| Number of reflections | 11363 | |
| <I/σ(I)> | 11.85 | 4.3 |
| Completeness [%] | 100.0 | 82.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | Crystals of 3D5/EE_48.K (7.5mg/ml in HBS) were grown at 4 C with 0.1M Tris pH 8.5, 0.2M Li2(SO4), 3% 6-aminohexanoic acid, 24% PEG 8000; crystals appeared in 4-5 days, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |
