4NKB
Crystal Structure of the cryptic polo box (CPB)of ZYG-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 200 |
| Detector technology | PIXEL |
| Collection date | 2011-09-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 53.383, 60.093, 87.515 |
| Unit cell angles | 90.00, 93.31, 90.00 |
Refinement procedure
| Resolution | 24.957 - 2.300 |
| R-factor | 0.2457 |
| Rwork | 0.243 |
| R-free | 0.27590 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.876 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| <I/σ(I)> | 1.53 | |
| Completeness [%] | 97.5 | 96.2 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 100 mM tri-sodium citrate (pH 5.6), 15% (w/v) PEG 4,000, 200 mM (NH4)2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
