4NK6

Crystal Structure of the periplasmic alginate epimerase AlgG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.075
Spacegroup name	P 32 2 1
Unit cell lengths	126.616, 126.616, 97.708
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.813 - 2.097
R-factor	0.1893
Rwork	0.188
R-free	0.21460
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.138
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.097	2.097
Rmerge	0.088	0.587
Number of reflections	53161
<I/σ(I)>		6.8
Redundancy		21.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.26 M (NH4)2SO4, 0.1 M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K