4NJN
Crystal Structure of E.coli GlpG at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Detector technology | CCD |
| Collection date | 2012-12-09 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 110.680, 110.680, 127.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.910 - 2.400 |
| R-factor | 0.19833 |
| Rwork | 0.196 |
| R-free | 0.24765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.965 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.910 | 2.320 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Number of reflections | 9974 | |
| Completeness [%] | 99.7 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 3M NaCl, 0.1M Sodium acetate, 10% Glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
