4NJM
Crystal Structure of phosphoglycerate bound 3-phosphoglycerate dehydrogenase in Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2011-09-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.718, 77.346, 89.925 |
| Unit cell angles | 90.00, 101.78, 90.00 |
Refinement procedure
| Resolution | 88.030 - 1.790 |
| R-factor | 0.20628 |
| Rwork | 0.204 |
| R-free | 0.24135 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nfy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.076 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.030 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Number of reflections | 63428 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 25% PEG 3350, 100mM Tris pH 7.0-8.0, 200mM sodium formate, 5% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 289K |
