4NJL
Crystal structure of middle east respiratory syndrome coronavirus S2 protein fusion core
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-02 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 43.510, 43.510, 129.633 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.180 - 2.300 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wyy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.537 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.180 | 2.370 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 6795 | |
| Completeness [%] | 99.3 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 289 | 0.1 M HEPES-NaOH, 25% (w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
