4NJ8
Crystal structure of the human ANKS3 SAM Domain L52A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 |
| Unit cell lengths | 71.890, 71.890, 33.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.890 - 1.600 |
| R-factor | 0.1995 |
| Rwork | 0.198 |
| R-free | 0.21770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.1) |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 7.150 | 1.600 |
| Rmerge | 0.084 | 0.106 | 0.777 |
| Number of reflections | 22798 | 278 | 1640 |
| <I/σ(I)> | 20.11 | 30.59 | 5.4 |
| Completeness [%] | 99.3 | 96.5 | 99.3 |
| Redundancy | 15.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 277 | 15% PEG-8000, 100mM sodium/potassium phosphate, 0.3M sodium chloride, pH 6.6, vapor diffusion, hanging drop, temperature 277K |
