4NJ5
Crystal structure of SUVH9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.2823 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.679, 84.197, 99.359 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.679 - 2.400 |
| R-factor | 0.1886 |
| Rwork | 0.187 |
| R-free | 0.22260 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.698 |
| Number of reflections | 21408 | |
| <I/σ(I)> | 35 | 3.5 |
| Completeness [%] | 98.3 | 100 |
| Redundancy | 14 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M potassium thiocyanate, 0.1 M Bis-Tris propane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
