4NJ0
GCN4-p1 single Val9 to Ile mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-23 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.199, 30.410, 27.788 |
| Unit cell angles | 90.00, 102.01, 90.00 |
Refinement procedure
| Resolution | 27.179 - 1.900 |
| R-factor | 0.2593 |
| Rwork | 0.258 |
| R-free | 0.28290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zta |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.657 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.960 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.045 |
| Number of reflections | 5462 |
| <I/σ(I)> | 20.4 |
| Completeness [%] | 99.3 |
| Redundancy | 3.29 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 0.2 M sodium acetate, 0.1 M sodium citrate tribasic dihydrate, 25% w/v PEG 4000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
