4NH6

Structure of human Dicer Platform-PAZ-Connector Helix cassette in complex with 15-mer siRNA having 5'-pUUU and UU-3' ends (2.55 Angstrom resolution)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	117.036, 84.126, 51.496
Unit cell angles	90.00, 109.67, 90.00

Refinement procedure

Resolution	33.666 - 2.551
R-factor	0.196
Rwork	0.193
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ngd
RMSD bond length	0.007
RMSD bond angle	1.089
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.550	5.490	2.550
Rmerge	0.073	0.042	0.449
Number of reflections	15309
<I/σ(I)>	10.4
Completeness [%]	99.5	98.9	96.7
Redundancy	4	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M sodium chloride, 0.05 M Tris-HCl, pH 8.3-8.8, 12-15% PEG20000, VAPOR DIFFUSION, HANGING DROP, temperature 293K