4NGZ

Previously de-ionized HEW lysozyme crystallized in 0.5 M YbCl3/30% (v/v) glycerol and collected at 125K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	125
Detector technology	IMAGE PLATE
Collection date	2000-05-17
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	78.589, 78.589, 37.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.570 - 1.700
R-factor	0.18081
Rwork	0.178
R-free	0.20515
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.011
RMSD bond angle	1.296
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.650	1.760
High resolution limit [Å]	1.700	1.700
Number of reflections	13242
<I/σ(I)>	23.3	3.8
Completeness [%]	99.7	97.7
Redundancy	9.4	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	Previously de-ionized lysozyme, no buffer added, 0.5 M YbCl3, 30% (v/v) glycerol , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K