4NGY
Dialyzed HEW lysozyme batch crystallized in 0.75 M YbCl3 and collected at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.666, 78.666, 37.151 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.630 - 1.350 |
| R-factor | 0.19304 |
| Rwork | 0.191 |
| R-free | 0.21586 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.697 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.180 | 1.430 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 48465 | |
| <I/σ(I)> | 12.8 | 2.9 |
| Completeness [%] | 99.1 | 98.5 |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Dialyzed lysozyme, no buffer added, 0.75 M YbCl3, pH 4.5, EVAPORATION, temperature 293K |
