4NGW
Dialyzed HEW lysozyme batch crystallized in 0.5 M YbCl3 and collected at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.665, 78.665, 37.129 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.620 - 1.370 |
| R-factor | 0.16495 |
| Rwork | 0.163 |
| R-free | 0.18766 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.493 | 1.450 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Number of reflections | 46325 | |
| <I/σ(I)> | 14.1 | 3.48 |
| Completeness [%] | 99.0 | 97.4 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Dialyzed lysozyme, no buffer added, 0.5 M YbCl3, pH 4.5, Batch crystallization, EVAPORATION, temperature 293K |
