4NGK
Previously de-ionized HEW lysozyme batch crystallized in 0.2 M CoCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-07 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.286, 79.286, 37.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.330 - 1.500 |
| R-factor | 0.12508 |
| Rwork | 0.122 |
| R-free | 0.15866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.341 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.330 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 19553 | |
| <I/σ(I)> | 36.6 | 4.1 |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 8.6 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Previously de-ionized lysozyme, no buffer added, 0.2 M CoCl2, pH 4.5, Batch crystallization, temperature 293K |
