4NGJ
Dialyzed HEW lysozyme batch crystallized in 1.0 M RbCl and collected at 100 K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.024, 79.024, 37.216 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.512 - 1.100 |
| R-factor | 0.14566 |
| Rwork | 0.143 |
| R-free | 0.17327 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.512 | 1.170 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Number of reflections | 90624 | |
| <I/σ(I)> | 16.5 | 3.2 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Dialyzed lysozyme, no buffer added, 1.0 M RbCl, pH 4.5, Batch crystallization, temperature 293K |
