4NGI
Previously de-ionized HEW lysozyme crystallized in 1.0 M RbCl and collected at 125K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 125 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-24 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.037, 79.037, 36.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.890 - 1.700 |
| R-factor | 0.17904 |
| Rwork | 0.176 |
| R-free | 0.21455 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.760 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 12958 | |
| <I/σ(I)> | 15.1 | 3.1 |
| Completeness [%] | 96.8 | 98.3 |
| Redundancy | 5.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Previously de-ionized lysozyme, no buffer added, 1.0 M RbCl, 30% (v/v) glycerol, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
