4NG1
Previously de-ionized HEW lysozyme batch crystallized in 1.9 M CsCl
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE D41A |
Synchrotron site | LURE |
Beamline | D41A |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2000-12-01 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 79.435, 79.435, 38.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.170 - 1.820 |
R-factor | 0.16081 |
Rwork | 0.158 |
R-free | 0.18954 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 193l |
RMSD bond length | 0.011 |
RMSD bond angle | 1.354 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.360 | 1.890 |
High resolution limit [Å] | 1.820 | 1.820 |
Number of reflections | 11429 | |
<I/σ(I)> | 12.7 | 3.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.5 | 293 | Previously de-ionized lysozyme, no buffer added, 1.9 M CsCl, pH 4.5, Batch crystallization, temperature 293K |