4NG1

Previously de-ionized HEW lysozyme batch crystallized in 1.9 M CsCl

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE D41A
Synchrotron site	LURE
Beamline	D41A
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2000-12-01
Detector	MAR scanner 300 mm plate
Spacegroup name	P 43 21 2
Unit cell lengths	79.435, 79.435, 38.115
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.170 - 1.820
R-factor	0.16081
Rwork	0.158
R-free	0.18954
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.011
RMSD bond angle	1.354
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.360	1.890
High resolution limit [Å]	1.820	1.820
Number of reflections	11429
<I/σ(I)>	12.7	3.9
Completeness [%]	99.9	100
Redundancy	6.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	Previously de-ionized lysozyme, no buffer added, 1.9 M CsCl, pH 4.5, Batch crystallization, temperature 293K