4NG0
Lar_0958 a cell surface adhesin from lactobacillus reuteri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2010-12-08 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.790, 36.990, 75.550 |
| Unit cell angles | 90.00, 100.64, 90.00 |
Refinement procedure
| Resolution | 29.014 - 1.501 |
| R-factor | 0.1592 |
| Rwork | 0.158 |
| R-free | 0.18720 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.988 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.777 | 46.777 | 1.540 |
| High resolution limit [Å] | 1.501 | 6.710 | 1.500 |
| Rmerge | 0.017 | 0.142 | |
| Total number of observations | 1562 | 13073 | |
| Number of reflections | 45978 | ||
| <I/σ(I)> | 30.1 | 40.4 | 5.5 |
| Completeness [%] | 99.4 | 81.4 | 97.8 |
| Redundancy | 4 | 3.3 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.2M Ammonium sulfate, 0.1M sodium acetate, 25% (w/v) PEG 4000, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
