4NFZ

Crystal structure of polymerase subunit PA N-terminal endonuclease domain from bat-derived influenza virus H17N10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-21
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61 2 2
Unit cell lengths	74.300, 74.300, 401.159
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.800 - 2.700
R-factor	0.242
Rwork	0.240
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2w69
RMSD bond length	0.013
RMSD bond angle	1.551
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.699	2.700
Rmerge	0.112	0.654
Number of reflections	19269
<I/σ(I)>	27.69	5.5
Completeness [%]	99.5	100
Redundancy	15.6	16.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	291	0.1M sodium chloride, 0.1M Bis-Tris, 1.5M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K