4NFY
Crystal Structure of 3-phosphoglycerate Dehydrogenase from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.15 |
| Detector technology | CCD |
| Collection date | 2011-09-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 |
| Unit cell lengths | 85.906, 85.906, 84.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.910 - 2.450 |
| R-factor | 0.19728 |
| Rwork | 0.194 |
| R-free | 0.25895 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wwk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.910 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Number of reflections | 22458 | |
| <I/σ(I)> | 36.4 | 7.4 |
| Redundancy | 4.2 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG, 100mM Tris-cl, 350mM sodium formate, 5% Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
