4NFW
Structure and atypical hydrolysis mechanism of the Nudix hydrolase Orf153 (YmfB) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-07-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97198 |
| Spacegroup name | P 41 |
| Unit cell lengths | 111.387, 111.387, 247.489 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.420 - 2.300 |
| R-factor | 0.24273 |
| Rwork | 0.240 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.781 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 128765 | |
| Completeness [%] | 96.8 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 283 | 1.4M ammonium sulfate, 0.1M MES buffer pH 6.0, 0.1M MnCl2, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
