4NFC

Structure of paired immunoglobulin-like type 2 receptor (PILR )

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-10
Detector	ADSC QUANTUM 315r
Spacegroup name	P 31
Unit cell lengths	42.803, 42.803, 130.205
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.650 - 2.200
R-factor	0.221
Rwork	0.219
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfb
RMSD bond length	0.021
RMSD bond angle	1.628
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.090	0.465
Number of reflections	13332
<I/σ(I)>	17.03	4.789
Completeness [%]	98.3	98.8
Redundancy	5.6	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5	291	3%(w/v) dextran sulfate sodium salt, 0.1M BICINE pH 8.5, 15%(w/v) polyethylene glycol 20,000, VAPOR DIFFUSION, SITTING DROP, temperature 291K