4NFA
Structure of the C-terminal doamin of Knl1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.933 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 74.855, 74.855, 88.756 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.413 - 2.497 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.24160 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.413 | 2.590 |
High resolution limit [Å] | 2.497 | 2.500 |
Number of reflections | 10352 | |
Completeness [%] | 99.8 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 120-300 KSCN, 100 mM BisTris Propane (pH 8.6-9.2), 17 % (v/v) PEG 3350 and 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |