4NEB
Previously de-ionized HEW lysozyme batch crystallized in 0.5 M MnCl2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-12-06 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.186, 79.186, 37.715 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.308 - 1.480 |
| R-factor | 0.12492 |
| Rwork | 0.122 |
| R-free | 0.15117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.308 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Number of reflections | 20575 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 293 | Previously de-ionized lysozyme, no buffer added, 0.5 M MnCl2, pH 4.5, batch crystallization, temperature 293K |
