4NCV
Foldon domain wild type N-conjugate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-06 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.970, 47.930, 27.970 |
| Unit cell angles | 90.00, 92.82, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.200 |
| R-factor | 0.1349 |
| Rwork | 0.133 |
| R-free | 0.16317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ncu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.233 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.032 | 0.495 |
| Number of reflections | 22182 | |
| <I/σ(I)> | 15.7 | 2.3 |
| Completeness [%] | 95.8 | 91.9 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.4 M Na/K-phosphate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
