4NCE

Influenza polymerase basic protein 2 (PB2) bound to 7-methyl-GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 65
Unit cell lengths	81.440, 81.440, 54.560
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.620 - 2.300
R-factor	0.19625
Rwork	0.195
R-free	0.22929
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vqz
RMSD bond length	0.011
RMSD bond angle	1.386
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	29.620
High resolution limit [Å]	2.300
Rmerge	0.134
Number of reflections	9272
<I/σ(I)>	8.3
Completeness [%]	100.0
Redundancy	6.45

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.7	298	0.7 uL of protein solution (9.7 mg/mL protein, 50 mM Tris buffer pH 8, 200 mM sodium chloride, 2 mM dithiothreitol, 5 mM m7GTP) was mixed with 0.35 uL Hampton Research Silver Bullet screening reagent #36. This mixture was combined with 0.35 uL of well solution (approximately 1.3 M sodium formate, 100 mM sodium citrate buffer pH 4.6, 5 mM dithiothreitol) and suspended over 1 mL of well solution, VAPOR DIFFUSION, HANGING DROP, temperature 298K