4NC8
N-terminal domain of delta-subunit of RNA polymerase complexed with nickel ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-18 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9871 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 39.462, 109.636, 82.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.500 - 2.170 |
| R-factor | 0.22682 |
| Rwork | 0.224 |
| R-free | 0.28263 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nc7 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.540 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 2.280 |
| High resolution limit [Å] | 2.160 | 2.170 |
| Number of reflections | 9786 | |
| Completeness [%] | 98.9 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290.15 | 2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K |
