4NC7
N-terminal domain of delta-subunit of RNA polymerase complexed with I3C and nickel ions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-21 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.5419 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 41.650, 110.200, 85.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.500 - 2.000 |
R-factor | 0.20418 |
Rwork | 0.201 |
R-free | 0.25937 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.731 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | Auto-Rickshaw |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.500 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 13651 | |
Completeness [%] | 99.9 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290.15 | 2M sodium/potassium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 290.15K |