4NC4

Crystal structure of photoreceptor AtUVR8 mutant W285F and light-induced structural changes at 120K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-05
Detector	RAYONIX MX-300
Spacegroup name	C 1 2 1
Unit cell lengths	111.798, 79.119, 189.340
Unit cell angles	90.00, 95.18, 90.00

Refinement procedure

Resolution	28.490 - 1.750
R-factor	0.15
Rwork	0.149
R-free	0.18400
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	4naa
RMSD bond length	0.006
RMSD bond angle	1.055
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.750
Rmerge	0.047
Number of reflections	164911
Completeness [%]	99.8
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9.2	290	protein concentration 8mg/ml, mother liquor 0.1M MgCl2 15-18% PEG8000 and 0.1M Tris HCl pH 9.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K