4NBW
Crystal structure of FabG from Plesiocystis pacifica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-09-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 77.730, 75.140, 97.725 |
| Unit cell angles | 90.00, 113.43, 90.00 |
Refinement procedure
| Resolution | 44.832 - 2.000 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q7b |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.334 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.832 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 61472 | |
| Completeness [%] | 87.9 | 87.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.2 M Ammonium tartrate dibasic pH 7.0 and 25 % PEG 3,350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
