4NB2
Crystal Structure of FosB from Staphylococcus aureus at 1.89 Angstrom Resolution - Apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.608, 44.825, 45.665 |
| Unit cell angles | 119.30, 106.79, 97.92 |
Refinement procedure
| Resolution | 36.800 - 1.890 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.027 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.3.0) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.800 | 1.940 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.343 | |
| Number of reflections | 18193 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 89.7 | 78.4 |
| Redundancy | 1.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | PEG 3350, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
