4NB1
Crystal Structure of FosB from Staphylococcus aureus at 1.80 Angstrom Resolution with L-Cysteine-Cys9 Disulfide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | Bruker Proteum PT135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.879, 44.924, 46.348 |
| Unit cell angles | 61.19, 65.04, 82.50 |
Refinement procedure
| Resolution | 39.190 - 1.800 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.957 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.190 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.225 | |
| Number of reflections | 23472 | |
| <I/σ(I)> | 2.73 | |
| Completeness [%] | 97.1 | 80.7 |
| Redundancy | 1.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 303 | PEG 3350, Ammonium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
