4NAS
The crystal structure of a rubisco-like protein (MtnW) from Alicyclobacillus acidocaldarius subsp. acidocaldarius DSM 446
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-13 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97941 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.763, 175.671, 83.653 |
| Unit cell angles | 90.00, 107.67, 90.00 |
Refinement procedure
| Resolution | 29.500 - 1.920 |
| R-factor | 0.1975 |
| Rwork | 0.195 |
| R-free | 0.25080 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.071 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.500 | 1.950 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.085 | 0.625 |
| Number of reflections | 113403 | |
| <I/σ(I)> | 22.8 | 2.3 |
| Completeness [%] | 96.3 | 94.2 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.2M CalCl2, 20%(w/v) PEG3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
