4NAO
Crystal structure of EasH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.24908 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 91.304, 91.304, 79.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.326 - 1.649 |
| R-factor | 0.1583 |
| Rwork | 0.157 |
| R-free | 0.18190 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.660 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 80988 | |
| Completeness [%] | 99.6 | 96.1 |
| Redundancy | 8.4 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 40% (v/v) PEG 600, 125 mM Na3citrate at pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
