4NAJ

Crystal Structure of Two-Domain Laccase from Streptomyces Lividans AC1709 in complex with azide after 90 min soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-08
Detector	RAYONIX MX-225
Wavelength(s)	0.91841
Spacegroup name	P 43 3 2
Unit cell lengths	177.020, 177.020, 177.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.097 - 2.600
R-factor	0.171
Rwork	0.169
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gyb
RMSD bond length	0.008
RMSD bond angle	1.170
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Number of reflections	29677
Completeness [%]	99.8	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	296	31%(v/v) PEG monomethyl ether 550, 0.1M NaCl, 0.1M glycine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K