4N8X
The structure of Nostoc sp. PCC 7120 CcmL
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.03322 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 117.880, 128.120, 220.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.292 - 1.930 |
| R-factor | 0.2077 |
| Rwork | 0.206 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n8f |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.486 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.292 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 234801 | |
| <I/σ(I)> | 16.6 | 2.2 |
| Completeness [%] | 94.0 | 65.1 |
| Redundancy | 4.8 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 273 | 0.1 M Na acetate, 20 % (vol/vol) PEG 8000, 0.1 M Li2SO4, 1 % ethylene glycol, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
