4N8U
Two-Domain Laccase from Streptomyces viridochromogenes at 2.4 A resolution AC629
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-09-25 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.720, 79.600, 204.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.950 - 2.400 |
R-factor | 0.1897 |
Rwork | 0.188 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tas |
RMSD bond length | 0.010 |
RMSD bond angle | 1.396 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.150 | 0.418 |
Number of reflections | 31212 | |
<I/σ(I)> | 7.98 | 2.63 |
Completeness [%] | 97.6 | 87.8 |
Redundancy | 3.45 | 2.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 285 | 11% PEG 4000, 0.1M Tris- l, 0.1M NaCl, 1.3% polyacrylic acid 5100 sodium salt, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |