4N8T
Human hemoglobin nitric oxide adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 53.567, 53.567, 192.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.480 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.25158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ird |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.876 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.500 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | |
| Number of reflections | 23142 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 6.8 | 295 | SOLDIUM PHOSPHATE, POTASSIUM PHOSPHATE, TOLUENE, HUMAN HEMOGLOBIN, pH 6.8, LIQUID DIFFUSION, temperature 295K |
