4N8I
M31G mutant, RipA structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.411, 107.171, 87.800 |
| Unit cell angles | 90.00, 120.36, 90.00 |
Refinement procedure
| Resolution | 43.748 - 2.011 |
| R-factor | 0.1366 |
| Rwork | 0.135 |
| R-free | 0.17990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qlk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.080 |
| High resolution limit [Å] | 2.010 | 4.330 | 2.010 |
| Rmerge | 0.089 | 0.055 | 0.293 |
| Number of reflections | 61943 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 99.6 | 100 | 96.5 |
| Redundancy | 3.6 | 3.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.2 M ammonium acetate, 24% PEG 3350 and 1 mM succinyl-CoA, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
