4N82
X-ray crystal structure of Streptococcus sanguinis NrdIox
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.078 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.726, 56.612, 73.387 |
| Unit cell angles | 90.00, 106.13, 90.00 |
Refinement procedure
| Resolution | 47.690 - 1.880 |
| R-factor | 0.2083 |
| Rwork | 0.207 |
| R-free | 0.23760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rlj |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.880 |
| Number of reflections | 33395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | PEG 4000, ammonium sulfate, pH 5.6, vapor diffusion, sitting drop, temperature 298K |
