4N7R

Crystal structure of Arabidopsis glutamyl-tRNA reductase in complex with its binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	59.368, 84.752, 359.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.557 - 2.802
R-factor	0.2145
Rwork	0.212
R-free	0.26980
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.020
High resolution limit [Å]	2.800	4.790	2.800
Number of reflections	43422
Completeness [%]	94.3	99	93.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	22.5%(w/v) polyethyleneglycol 3350, 0.3M ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K
1	VAPOR DIFFUSION, SITTING DROP	7	289	22.5%(w/v) polyethyleneglycol 3350, 0.3M ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K
1	VAPOR DIFFUSION, SITTING DROP	7	289	22.5%(w/v) polyethyleneglycol 3350, 0.3M ammonium citrate tribasic, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K