4N7L

Zinc Substituted Reaction Center M(L214H) Variant of Rhodobacter sphaeroides

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12709
Spacegroup name	P 31 2 1
Unit cell lengths	139.590, 139.590, 184.052
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.570 - 2.850
R-factor	0.1672
Rwork	0.165
R-free	0.21570
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.013
RMSD bond angle	2.652
Data scaling software	SCALA (0.1.27)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	120.888
High resolution limit [Å]	2.850
Rmerge	0.167
Number of reflections	42741
<I/σ(I)>	12.6
Completeness [%]	87.4
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1