4N7B
Structure of the E-1-hydroxy-2-methyl-but-2-enyl-4-diphosphate reductase from Plasmodium falciparum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2010-01-01 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 115.960, 115.960, 76.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.048 - 2.198 |
| R-factor | 0.1728 |
| Rwork | 0.172 |
| R-free | 0.19490 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.132 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.048 | 2.300 |
| High resolution limit [Å] | 2.198 | 2.200 |
| Number of reflections | 30552 | |
| Completeness [%] | 99.5 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 30 % w/v PEG 4000, 100 mM sodium citrate (pH 5.6), 100 mM ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
