4N75

Structural Basis of BamA-mediate Outer Membrane Protein Biogenesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	118.492, 159.883, 56.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.268 - 2.604
R-factor	0.2081
Rwork	0.205
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k3c
RMSD bond length	0.010
RMSD bond angle	1.421
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	35.270	3.450
High resolution limit [Å]	2.604	6.260	2.600
Number of reflections	31883
Completeness [%]	93.5	88.7	96.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	289	0.1M MES PH 6.0, 6% Tacsimate, 25% PEG 4000, 0.6% C8E4, VAPOR DIFFUSION, HANGING DROP, temperature 289K