4N6U
Adhiron: a stable and versatile peptide display scaffold - truncated adhiron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-09 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 41 |
| Unit cell lengths | 36.373, 36.373, 59.243 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.373 - 2.251 |
| R-factor | 0.1866 |
| Rwork | 0.184 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.284 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.102 | 0.722 |
| Number of reflections | 3709 | |
| <I/σ(I)> | 8.2 | 1.8 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 280 | PACTpremier (D02), 0.1 M MMT System pH 5.0, 25.0% w/v Polyethylene Glycol 1500, VAPOR DIFFUSION, SITTING DROP, temperature 280K |
