4N6S
Crystals of cross-linked stabilized and functional Phycobilisomes: only phycocyanin rods contribute to diffraction.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97950 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 188.070, 188.070, 60.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| Rwork | 0.192 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o18 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.300 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.312 |
| Number of reflections | 16012 | |
| <I/σ(I)> | 20 | 6.3 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 4.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 10-15 mg/ml phycobilisome in 0.9M phosphate buffer against a resevoir of 1.2-1.4M phosphate buffer. , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
