4N6J
Crystal structure of human Striatin-3 coiled coil domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 1W2B |
| Synchrotron site | BSRF |
| Beamline | 1W2B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-09-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 34.064, 34.064, 161.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.500 - 2.001 |
| R-factor | 0.2085 |
| Rwork | 0.203 |
| R-free | 0.25210 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.924 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.041 | 0.083 |
| Number of reflections | 8009 | |
| <I/σ(I)> | 43.11 | 24.19 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 16.2 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1M Bis-Tris pH 6.5, 0.2M MgCl2, 23% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
